Workshops disponíveis:
The Power of Microscopy in Research
Realizado por:
Daniela Ferreira, Sandra Louzada e Mariana Lopes
Daniela Ferreira, Sandra Louzada e Mariana Lopes
Microscopy constitutes an important technique used to visualize and study cellular structures and their function. Over the years, several types of microscopes have been developed to meet investigative demands, allowing to observe fixed or live cells, chromosomes, and specific cellular components. One of the main advantages of such technique is the ability to provide single-cell analysis, essential for data validation and complementation of results obtained with other methods. Moreover, the detailed cell-to-cell analysis provided by microscopy, allied to the use of specific imaging softwares, has contributed to a great advance in biological research.
This workshop will consist in a brief theoretical introduction to some concepts of microscopy and its various applications in biological research, followed by a hands-on approach where the participants will have contact with different types of microscopes and applications as well as training in softwares for image analysis.
This workshop will consist in a brief theoretical introduction to some concepts of microscopy and its various applications in biological research, followed by a hands-on approach where the participants will have contact with different types of microscopes and applications as well as training in softwares for image analysis.
Breaking the code –bioinformatic tools in cancer research, diagnosis and treatment
Realizado por:
Ana Escudeiro e Filomena Adega
Ana Escudeiro e Filomena Adega
In recent years, scientific research has revealed an increasing number of variants associated to different diseases. Particularly in cancer, these mutations can be used in diagnosis, prognosis, therapeutic decision, follow up of patients and risk population assessment. Its identification is thus a very important and valuable tool in the fight against this group of diseases. Sequence technologies as Sanger and Next-generation sequencing are widely used for mutation detection in clinical settings. But the analysis relies on a strong bioinformatics approach, where the data generated from sequencing technologies is a critical process involving base calling, read alignment, variant identification, and annotation. During this process, the sequence information is compared to a reference to identify whether there are any variants in the targeted sequences. The annotation and interpretation processes are then set to identify and classify each variant and their clinical significance. In the present workshop, the participants will be challenged to solve clinical cases, identifying the presence/absence of genome mutations through the analysis of sequence data using bioinformatics’ software and web-based tools. The exploration in databases and genome browsers will allow the interpretation of the detected mutations and the discussion of disease diagnosis, prognosis, and therapeutics.
Keywords: Bioinformatics, Variant analysis, Diagnosis, Prognosis, Case studies
Keywords: Bioinformatics, Variant analysis, Diagnosis, Prognosis, Case studies
DNA & RNA Biosensing platforms
Realizado por:
Paula Lopes e Helena Gonçalves
Paula Lopes e Helena Gonçalves
There are several biosensing platforms used in our daily lives, based on the detection of different types of analytes. In this workshop, we will focus on the platforms that aim to detect nucleic acids, and we will explore the various alternatives that have been reported in the literature. The different areas of applications will be addressed, as well as the potential that these systems have in each sector. We will end with the demonstration of a biosensor developed by a consortium between UTAD, REQUIMTE and ISEP in the context of the SARS- CoV2 testing.
Research funding: Cdots Biosensing COVID19, nº 041_596518523, financed by RESEARCH COVID-19 of the Portuguese Foundation for Science and Technology.
Research funding: Cdots Biosensing COVID19, nº 041_596518523, financed by RESEARCH COVID-19 of the Portuguese Foundation for Science and Technology.
SSRs for genotyping plant genetic resources
Realizado por:
Márcia Carvalho e Isaura Castro
Márcia Carvalho e Isaura Castro
This workshop intends to provide an overview of the application of the SSR molecular marker for the plant genetic diversity assessment, specifically in species belonging to genus Vigna. During the workshop will be addressed the theoretical concepts of SSR molecular marker, its applications to plant breedings and management of germplasm collections. In the practical part, the participants will have the opportunity to analyze SSR genotyping data in species of the genus Vigna with specific softwares, identify varieties and determine relationships between them and prepare dendrograms.
Collection and preservation of samples in forensic science- from necropsoy to lab
Realizado por:
Isabel Pires e Anabela Alves
Isabel Pires e Anabela Alves
A post-mortem examination is essential to identify the cause and circumstances of death. Even in the age of molecular pathology, necropsy remains the most valuable tool for understanding the organism and the disease. Forensic science is about using scientific knowledge to support the application of the law. However, for the evidence to be valid, the sampling and preserving samples must consider some rules. In fact, the forensic investigator's biggest nightmare is that the evidence will not be admissible in court.
In this workshop, we will perform necropsies of various animal species to collect samples for further forensic analysis: genetics, toxicology, ballistics and histopathology
In this workshop, we will perform necropsies of various animal species to collect samples for further forensic analysis: genetics, toxicology, ballistics and histopathology
Silk, a biopolymer as material for flexible sensors
Realizado por:
Guilherme J.G. Sousa, Tiago A.G. Duarte and V. de Zea Bermudez
Guilherme J.G. Sousa, Tiago A.G. Duarte and V. de Zea Bermudez
Nature is an inexhaustible source of inspiration for the creation of innovative materials and devices with improved performance. In this context, silk appears, a biopolymer with a millennial history in the textile and biomedical industry. The extraordinary intrinsic characteristics of silk fibers, such as "self-assembly", biocompatibility, non-toxicity, superior mechanical properties and piezoresistivity (change of resistance that can be measured on physical contact), among others, offer a wide range of potential application in technological areas of the greatest relevance, such as optics, electronics and energy.
This workshop intends to provide an overview of silk science and highlight the tremendous technological potential, namely on sensors field. The basics of silk processing follow by a silk film synthesis and sensor circuit printing will be presented and explored in practical laboratory experiments. The participants will perform degumming of silk fibers harvested from Bombyx mori silkworm cocoons and obtain a silk-based film. The final step will consist of a demonstration on printing of the electronic sensor circuit on a silk film.
This workshop intends to provide an overview of silk science and highlight the tremendous technological potential, namely on sensors field. The basics of silk processing follow by a silk film synthesis and sensor circuit printing will be presented and explored in practical laboratory experiments. The participants will perform degumming of silk fibers harvested from Bombyx mori silkworm cocoons and obtain a silk-based film. The final step will consist of a demonstration on printing of the electronic sensor circuit on a silk film.